Always test what you uploaded on itch, because that's not how you upload a game.  Just the executable is not enough.

Sorry we can't play it, good luck next jam

Hi, it looks like you only included the unity executable itself, but not the rest of the game files required to run it. You should also include a folder called "Built To Scale_Data" and a "unityplayer.dll" file together with your executable (in a zip file) in order for other people to be able to play your game.