You are right. I didn't adjust the grid size for the title image to 400 x 300. Thanks! I will check the differences between your project.zip folder and what I wrote in my project.
With the start buttons, I could not find anything in the tutorial about what to adjust the width and height to. There is a brown square underneath startoff.png (see attached image here). As you can see it overlaps on the player 2 side of text on the word "Up". And also with the start button, the script is just like yours in the tutorial, but when my mouse cursor hovers over it or clicks the button it does not do anything.
Sorry for the trouble and thank you for your help! I really appreciate it :)