There has been a change with the itch.io launcher that is causing the game to run natively in 2D. If you open the game by way of the Holodexxx.exe, it will run in VR if you have SteamVR running.
Sorry for the annoyance, we are trying to figure out this issue as well.
Viewing post in Holodexxx: Experimental 2020 comments
Having this issue as well, if a fix can be detailed in the Help or faqs section would be great since I suspended my pledge till I can get the VR mode working, if it doesn't then it goes against why I even invested in it. So please lay out a user-friendly fix for everyone so we know. Even exiting steam VR and going Virtual Desktop didn't work everything is running in 2D regardless of which file I run first etc.. Cant see any button to change to VR either or change setup to run VR default.