What I'm trying to say is that if the girls are virgin or not, or with who lose their virginity is completely useless to the story, and the topic WILL NEVER be mentioned. So, I'm saying that if you want to know if a girl is a virgin or not, you have to judge that for yourself. Most girls will explicitly say they are virgins, others not.
And yes, you can freely imagine what girl is not a virgin and with who, but it won't be EVEN HINTED in the story, even, I think those characters as "They are not virgin. Period.", with who? I don't know.