First off I haven't seen this line yet. I'm jealous of you. Secondly I agree. I would like to see the Wawsp Queen's soft side once in a while.
I have not committed a mass-harm of the larvae in that room. I can never bring myself to do it; I just don't want to hurt those babies. Again I do opine that you're right that if the larvae are hurt the Queen should be devastated. That being said, I also opine there should be lines added for even if you don't hurt them. The Queen could comment on what it's like to raise them; what their names are; what they like to eat; what she hopes they grow up as.
I for one would even like a special line or two if you go into that room and then leave, either on foot or via portal, without hurting any of the larvae. Perhaps something like "You spared my babies. I didn't expect that. So, uh... thanks."