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Still no luck with latest version, game fails to load displaying the same error from original post.

Nope, same error.

Does the same when run as administrator.

Win10 

___________________________________________
############################################################################################
FATAL ERROR in
action number 1
of  Step Event0
for object obj_loading:

Variable obj_loading.item(100109, -2147483648) not set before reading it.
at gml_Script_load_items
############################################################################################
--------------------------------------------------------------------------------------------
stack frame is
gml_Script_load_items (line -1)
gml_Object_obj_loading_Step_0