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In Unity you need to create: New > Material, then choose Sprites/Difuse, set the color to pure black and add it to every single object to make it hide when no light is over it. Then, you need to add a source of light to your hero. Check this out: https://manygames.itch.io/thestrangebob
There is a hidden area to the left, destroy the boxes to get access. There, you will find a tower, inside this tower every object has this material.

What IS UP, fellow!?

Just passing to share with you a small prototype of mine using your amazing tileset: https://manygames.itch.io/thestrangebob

I'm still learning the unity engine, with zero experience on how to design levels so, hope you enjoy and don't get mad if i literally copy those 4 slices of scenario you have here in this page :D.