The Binding of Isaac fangame, heavily inspired by Plants vs. Zombies · By DoctorHummer, toncho_
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No, it does not. When you start a challenge, your current run's data can still be continued from. When the challenge ends in anyway, you can play your other run
so its safe to do challenges cuz you keep the current run
As weewoooweeewooo said, challenges won't take up your run since they're a single level anyway.
