Spine Vtuber Prototype uses these animations:

1. breathe
2. face pitch down
3. face pitch up
4. face roll left
5. face roll right
6. face yaw left
7. face yaw right
8. idle
9. left brow raise
10. left eye open
11. left pupil pitch down
12. left pupil pitch up
13. left pupil yaw left
14. left pupil yaw right
15. mouth height
16. mouth width
17. right brow raise
18. righteye open
19. right pupil pitch down
20. right pupil pitch up
21. right pupil yaw left
22. right pupil yaw right

All these animations need to be created for the model for it to work in Spine Vtuber Prototype. If you are missing any of the required animations, Spine Vtuber Prototype will pop-up alert you about it. You can set any FPS and create multiple keyframes on the timeline to represent the range of movement. You want to to animate within one second. Any animation past one second could be ignored by this application. For example, if you set your Spine file for 60 FPS, you should not try to animate past 60 frames.