I like this a lot, you've definitely got the chiptune style down very well, there are some really nice catchy melodies and parts.
For the most part everything sits very nicely together and sounds well balanced, a couple moments here and there where an element maybe felt a little bit too loud but only a touch and mostly everything was just right.
I would say Funky Files could have used a very short lead in at the start, did get a little bit of a jump scare from it haha.
There's a good variety of different sounds and styles here too whilst still feeling very coherent and I like the moments where you've chosen to use a little hint of more modern production styles and techniques to elevate the track but without having it lose that retro feel.
I think my favourites were:
Bustling Brain, that had a real nice underlying groove with the drums, bass and piano and I really liked the piano solo.
Also really liked Overpowering Emotions, short but really full of emotion executed in a kinda jazzy way I really liked.
At first I was thinking Battle Against A Traumatic opponent was maybe too upbeat, even within the context of the soundtrack, but as it went on I think you really got across that feeling very effectively.