I'll see if I can find a solution but right now, I have no idea. :/ Didn't change the way I upload my builds since the first chapter. Are on Mac or PC ?
When I download an itch.io game, I basically run the .exe file located inside the folder, it looks like you do it another way ?
Also make sure to delete your previous files (pinned a topic about it) as a lot from Chapter 1 as changed. Running it as it is will be extra buggy.
I'll try to search for a solution. Keep me updated.