When I try to do Unity sprite editor and slice it into 16x16 pixel, it seems to be off. Any recommendations on settings I need to modify?
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I'm not an expert with Unity by any means, and haven't used Unity for a long time. I'd recommend asking the Unity community about this.
There shouldn't be any offsets you need to make (the tiles are all aligned correctly), however there are issues with the 2D tile system in Unity and tearing, which might be what you're encountering. Again, I'm not an expert in Unity, so I'd recommend asking there about these problems.