The only thing i really want to change with my game (that would make it 2 handed) is to add aiming with the mouse instead of Auto-Aim.
I asked one of the moderators on the discord and they said:
"sure, you can do that. make sure your submission follows the limitation though. you could make web build one hand control and then include a downloadable with option to have classic controls"
after the jam ends, I'll make the non-jam controls the default one (that plays in browser)