There's a lot of things going on here but thankfully they all work well! I don't often play clicker games and sadly this one does fall into the same trap; after playing for a while there's not enough new things happening to keep you engaged.
There were two difficulties but maybe you should try increasing the difficulty over time, or perhaps even a shop to buy more powerful knives and such; at least this would provide a clear goal to work towards. I noted that you referred to this as a "Psychological Horror". Perhaps I missed something, or I'm just not scared enough of spiders to understand it, because I was not at all scared when playing.
The audio was decent and did indeed fit the retro graphics style you chose, but it did seem to loop a bit too fast. Besides that, I thought that the thumbs down/thumbs up system was a really good idea as some reflex based gameplay was enough to keep me interested enough in playing until I died. There's a good use of the theme "reusable" too, but I think you could do without being able to buy a new knife; it sort of makes the ability to fix the knife less important.
Even though the game had no tutorial, I understood the instructions on the game page well enough to work out what I was doing. I've learned one thing from this game about killing spiders, eating spiders and fixing knives: it's a process.
Nice game, and I'll surely play again if you choose to update it in the future.