Well this is a bit of a strange one, we have tried both re-testing the build in our local version of the game, downloading it here from Itch, and making a new build, and cannot replicate this issue.
The only things I could think would be that perhaps you are not unzipping all of the files, you could be moving the files to a location but not all of the files to the same location, or you are missing some obscure redistributable files (but then you would be having similar issues with other Unity based games).
The game is also 64-bit only now so if you are trying to run this on a 32-bit machine it wouldn't work at all, but again we can't seem to recreate the issue and it's a different issue than what some other people here have found and what I (think) we have fixed previously.
Could you upload and send a link with the report log?