I liked the genre you've gone for considering the jam constraint, and I always liked these skill-based games!
The main problem I had with this game was that the buttons I needed to press were arrow keys, but the locations those buttons relate to are in a horizontal layout. I think numbers (1, 2, 3, and 4) would fit the game more controls-wise since they are in a horizontal layout, unlike arrow keys.
Good job on tweaking with Python!