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There is a RPGmaker autotile importer for unity to import tileset from RPGmaker. But you could also change the grid size to 24px for using the autotiles.

Because I am not familiar with RPG Maker, do I need to use 24*24 segmentation for the remaining tiles except A1 to A5?

A5 are simple 48px textures. You don't need a change there. A1 is animated water and also a autotile except the waterfall. You can split the waterfall vertical 3 times for a smooth animation. I hope I could help.