Each route has its own events, scenes, animations, etc. For example, on Ash's love route, you will have the sinners' room open, while on the slave route, you won't. Also, in the Waifu House, the secondary character on the Ash's love route is Asuna from SAO, and on the Ash's slave route, there is Mikasa from Attack on Titan. But Mikasa appears in version 0.3.1.