Spacebar is no longer the default hotkey for playing/pausing video as per the latest update (it is now the pause button on a keyboard, but you can rebind it if you don't have that key or would prefer a different one).
"Set app to capture hotkeys" has been removed because it was not useful enough to stay.
All hotkeys work when the window is unfocused, so I recommend picking hotkeys that don't conflict with your animation program :) This makes it fast to control Animation Buddy.