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Monster Queen (IIRC, she's called the Frost Queen or something?) 

She's the antagonist of the Intelligent Monster's route, which is insanely stupid to access.

To access it:

-You must have Julia

-Julia must have Detect  IIRC (1200 Support proficiency - yes, you read that right. Have fun...)

-Julia must be in her Normal Personality

-Julia must be part of your active party (meaning you bring her to the dungeon).

-IIRC, you must have obtained the Berserk spell for Ashley (level her a LOT - try being level 25-30 at minimum).

Lose 2 battles against any 2 random mobs that don't give a unique ending (for example, Baby Slimes). 

Lose 2 battles against 2 Monster Girls (Flower Girls, Slime Girls etc). 

Enter the dungeon with a party consisting of the Protagonist, Julia and a third member who is not offensive (so you can't bring Val, Sarah or Derrick for example - Cass or Molly is fair game to bring). 

Wait around and see a scene in which Julia notices monsters following you. Choose either option.

I forgot how long it's after that, but I think it's 3 more scenes (I could be wrong on this), a boss fight will occur... during which you only have access to Ashley. Who you can't control. Because she's permanently in her Berserk state. Which is why I told you to level her a ton. 

There's four possible outcomes:

-You lose: game over. 

-You lose: Julia has her gun from the Julia's Arsenal route. Julia shoots the Frost Queen and saves the day. It does not matter if Julia got help from Brittany or not.

-You win: Frost Queen gets her neck broken by Ashley (automatic if you win).

-You win: Frost Queen gets enslaved by you (there's like 3 lines total where you can cast a Chakram on the Frost Queen after realizing she's pretending to be dead). This unlocks her in the Monster Ranch for your amusement.