Your problem, as I understand it from your description and some guess working, is either:

• You uploaded Room222 successfully, it runs on your test system, but it won’t run on itch.io.
• You uploaded another game, but it is showing up nowhere on itch.

.

• If it’s the first, I would suggest downloading and testing the code and maybe reading the HTML5 FAQ again. Double-check if all the files (including the index) are in the right places.
• If it’s the latter, try to contact the support team.

Additionally, you could try the forum search. You problem might be more common than you think…