EDIT: It seems to be related to the browser, somehow? I used the Brave browser, and I get the same 188 tile count as GB Studio. It's just Firefox giving me the incorrect count. I'm not sure how that works, but just an FYI for anybody else experiencing this. Try a different browser.
I was doing some last minute edits to my submission before the deadline, and the online tile counter is telling me tiles are different when they are identical. It's saying I have 206 unique tiles. But if I load the exact same image into GB Studio, setting the pallete and everything, it says I have 188 unique tiles. I even skimmed through the heatmap and visually verified it was duplicating identical tiles. I even went and copy-pasted one of the tiles over, thinking maybe there were a couple of pixels just slightly off, but I get the same result. I'm going to assume, since GB Studio is what's going to be used to generate the rom, that I can go by the GB Studio count?