Music is very nice - even though it's repetitive, it does work well with the game. I also like that graphics are kept in same manner for most of the game.
I made to the boss, but I must say, I had hard time killing it. Specially when queen was spawning those crabs, which I consider to be too OP. I also had really hard time avoiding/blocking those homing missiles - not sure if there is any way to block 100% damage from that.
I would maybe change the slow attack with the quick one (shift to use slow, quick as default), as I almost always took at least one hit from ant. Maybe increasing player's range would help that too.
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Thanks for the compliment on the music and art style!
As for the boss, If you click levers in the boss room you regenerate health during the attack.
To nullify homing missiles - run them into the boss or other enemies or reflect them into the wall.
And as for the attacks - I originally had them switched, but the slow attack I found to be more useful against flying enemies and OHKO enemies (like the beetle and butterfly later on). Higher range could be a good idea though. Or maybe just keybinds so you can control the attacks.