For this jam I used a mix of Pyxel Edit and Photoshop CSS.
I've actually used Asesprite for some previous game jams, but I always come back to Photoshop, and recently have started using Pyxel Edit for animation.
Thank you! I'll take a look at Pyxel Edit. Seems pretty nifty for larger-scale things.
Pyxel Edit is great for animation, but I still use Photoshop for mockups and anything that isn't just single assets.