I wonder if that's the guinea pig voice files. Glad you liked it!
I'll see if anyone else has problems with Twitch on the RenPy forums - it's possible Twitch was using the window ID for the loading screen, which, of course, wouldn't exist once everything has loaded.
It may be that it can't handle OpenGL 3. Holding SHIFT when double-clicking/RETURN on the executable will bring up the game preferences. It may be worth trying AGILE first