Great to see a rhythm game in jams. Definitely hard to incorporate the musical scale theme , so I'm glad someone takes a crack at it. Multiple music choice was nice to reach a wider audience.
I'm guessing you forgot to use time.delta time when coding the speed ,as high framerate increased the difficulty quite a bit :P
only suggestion is maybe make the falling notes the same direction arrow as what they would fall over instead of circles . I would watch the top as they fell and then forget which lane was which