A still 160 x 144px image - could be a work of art, an example screenshot of a game, a cut scene, pure black image, a star field, checker-board, the Mona-lisa, Sonic beating up Mario, an example game HUD, etc.
Anything that fits the theme(s), and is 160 x 144, and no more than 192 unique 8x8px tiles (counting tiles).