Hi
To your question.
I always do everything in a 48x48 grid. After creating it in a 48x48 grid, I put it in 768x768 for rpg maker tilesets. The animation trees are mostly in 154x158 dimensions, the scale can be better there. The characters are 48x96 and put in rpg maker 576x768. Everything is made for rpg maker but approximately 2x scale should be ok.
I use 2k screen 2560x1440
If you use a 4k screen, I don't know how the pixels look on such a screen when they are scaled.
scale 2x -from solar colony
It's fine for larger props, but it's worse for small props.
If you have any questions, I will be happy to answer them.